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To Sell Keladi tikus - penawar kanser

cure4kanser

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UNTUK MAKLUMAT LANJUT LAYARI WWW.CURE4KANSER.TK

:)cgrock
 
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tokputih

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bolehler komersialkan.. pasti untung riban2
 

ed_zero

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wao..bgs ni..leh tanam kat dpn umah..hehe..:)cgrock
 

cure4kanser

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cuba tanam depan rumah anda semua dan cuba dapatkan kelulusan kementerian kesihatan malaysia lepas tu bolehlah nk cakap ape pon.tima kasih....
 

THX

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naper takde penjelasan secara saintifik, bagaimana keladi ni bertindak membunuh sel kanser?
Seingat saya, tumbuhan yg pahit seperti ubikayu, apricot, keladi mempunyai vitamin B17 yg berupaya membunuh sel kanser. Tapi ada side effect kalau makan begitu saja. Wallahualam.
 

cure4kanser

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Journal of Ethnopharmacology 118 (2008) 14–20
Contents lists available at ScienceDirect
Journal of Ethnopharmacology
journal homepage: www.elsevier.com/locate/jethpharm
Typhonium flagelliforme inhibits cancer cell growth in vitro and induces
apoptosis: An evaluation by the bioactivity guided approach
Choon-Sheen Lai a,c, Rosemal H.M.H. Masb, N.K. Nair a,
M.I.A. Majid c, S.M. Mansora, V. Navaratnama,c,∗
a Centre for Drug Research, Universiti Sains Malaysia, 11800 Penang, Malaysia
b School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
c Malaysian Institute of Pharmaceuticals and Nutraceuticals, Ministry of Science Technology and Innovation,
Universiti Sains Malaysia, 11800 Penang, Malaysia
a r t i c l e i n f o
Article history:
Received 14 September 2007
Received in revised form 22 February 2008
Accepted 29 February 2008
Available online 18 March 2008
Keywords:
Typhonium flagelliforme
Anticancer
Growth inhibitory effect
Cell line specific
Apoptotic
Chemical constituents
a b s t r a c t
Aim of the Study: Typhonium flagelliforme (Lodd.) Blume (Araceae) is a Malaysian plant used locally to
combat cancer. In order to evaluate its antiproliferative activity in vitro and to possibly identify the active
chemical constituents, a bioactivity guided study was conducted on the extracts of this plant.
Materials and Methods: The active extracts of Typhonium flagelliforme were fractionated by flash column
chromatography and each fractionwas evaluated for antiproliferative activity using MTT assay. The apoptotic
effect of the active fraction was determined microscopically and by using TUNEL colorimetric assay.
GC–MS and NMR were used to determine the chemical constituents of this active fraction.
Results: Several fractions of the hexane and dichloromethane extracts were found to inhibit the growth of
NCI-H23 non-small cell lung carcinoma cell line significantly, with IC50 <15g/ml. However, most of these
active fractions were also found to inhibit the growth of non-tumorigenic BALB/c 3T3 mouse fibroblast
cell line except for fraction 21 of the dichloromethane extract (D/F21). This particular fractionwas not only
less cytotoxic to the non-tumorigenic cells, where the IC50 was 48.6g/ml compared to IC50 7.5g/ml
for NCI-H23, but it was also found to induce apoptosis in the cancer cell line. GC–MS analysis revealed
that D/F21 contains hexadecanoic acid, 1-hexadecene, phytol and a derivative of phytol. The presence of
non-saturated fatty acids in this fraction was confirmed by nuclear magnetic resonance spectroscopy.
Conclusions: D/F21 was found to be the active and cancer cell line specific fraction of Typhonium flagelliforme.
Its major chemical constituents had been determined spectroscopically.
© 2008 Elsevier Ireland Ltd. All rights reserved.
1. Introduction
Typhonium flagelliforme (Lodd.) Blume (Araceae) is a herbal plant
which grows up to 30cm in height. It has an oblong whitish tuber,
triangular leaves and a spathe which is dilated and round at the
base enclosing the yellowish spadix (Ridley, 1967; Hsuan et al.,
1998). This plant grows wild inwasteland and is native to the South
East Asian countries and the southern part of India and Sri Lanka
(Nicolson and Sivadasan, 1981).
Typhonium flagelliforme, previously mistakenly referred as
Typhonium divaricatum (Nicolson and Sivadasan, 1981), is commonly
known as Rodent Tuber in Malaysia. Being described
ethnomedically as toxic, warming and phlegm resolving, this plant
is used to soothe swelling, coughing and more predominantly for
∗ Corresponding author at: Centre for Drug Research, Universiti Sains Malaysia,
Gelugor, 11800 Penang, Malaysia. Tel.: +60 4 6552829; fax: +60 4 6577957.
the treatment of cancer (Teo and Ch’ng, 1999; Lee andWong, 2004).
As a general practice, the juice of the fresh whole Typhonium flagelliforme
plant is prepared in honey to be consumed as a drink
(Teo and Ch’ng, 1999). There are also other practices where the
leaves are wrapped in Longan flesh and taken raw (Lee and Wong,
2004).
Several chemical constituents had been identified from Typhonium
flagelliforme. The hexane extract was reported to contain
saturated hydrocarbons and aliphatic acids (Choo et al., 2001a),
while the ethyl acetate extract was found to contain aromatic fatty
acids (Chen et al., 1997). No biological activities were indicated for
these compounds. In addition, phenylpropanoid glycosides, sterols,
and a cerebroside which has antihepatotoxic activitywere reported
from the root of this plant (Huang et al., 2004).
The polar extractswere investigated in vivo and found to be able
to ease expectoration, it is also antiasthmatic, anti-inflammatory,
analgesic and sedative (Zhong et al., 2001). Pharmacological studies
conducted on rats also indicated that the juice extract was able to
prevent hepatocarcinogenesis (Karuppiah et al., 1999).
0378-8741/$ – see front matter © 2008 Elsevier Ireland Ltd. All rights reserved.
doi:10.1016/j.jep.2008.02.034
 

maibee71

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naper takde penjelasan secara saintifik, bagaimana keladi ni bertindak membunuh sel kanser?
Seingat saya, tumbuhan yg pahit seperti ubikayu, apricot, keladi mempunyai vitamin B17 yg berupaya membunuh sel kanser. Tapi ada side effect kalau makan begitu saja. Wallahualam.

Bukan tumbuhan pahit tapi biji yg ada dalam tumbuh tumbuhan tertentu yg mempunyai kandungan tinggi B17 yg mampu membunuh sel cancer.
 

maibee71

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B17 ni memang dah lama digunakan di Hope Hospital Mexico untuk merawat pesakit cancer.
 

maibee71

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Kalau dilihat dari segi statistik memang jauh lebih berkesan daripada cara perubatan conventional moden kemo.
 

maibee71

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Tapi apa2 hal pun yg tentukan semua ni hanya Allah. Berubat merupakan jalan ikhtiar.
 
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